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Culture Lab Phone # : 1-877-572-4115 or 717-665-1274
Real-Time Q
PCR-based
DNA Mastitis Testing
What is PCR-based Mastitis Testing?β-lactamase penicillin resistance gene.PCR is a technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of a particular DNA sequence.
The real-time PCR-based mastitis assay is a revolutionary method for cow mastitis testing.
The assay can identify and quantify 12 major mastitis-causing species or groups plus Staphylococcal
What are the advantages?Samples do not need to be sterile
The preserved samples from Lancaster DHIA testing can be used
You dont need to collect a separate sample
You can also pool samples from groups of cows to help cut down on costs.Other advantages include selecting only your high SCC cows after they have been tested in the Lancaster DHIA lab. You can also tell your technician that you want all cows over a certain level of SCC tested. Plus, you can request testing of any fresh cows from the past month to help prevent a new case of mastitis.
We will send results to both you and your veterinarian aiding in more efficient treatment and decreased milk loss.
Why use PCR-based Mastitis testing over the standard culture?In approximately 2540 % of bovine milk samples taken from animals with clinical mastitis, no bacterial growth can be detected in conventional culturing.
PCR-based mastitis testing leads to more effective treatments by:
More specifically identifying coliforms
Identifying sources of mastitis in a single cow or bulk tank sample
Identifying sources of mastitis in cows already being treated
Decreasing test time from 2-7 days (conventional culturing) to 1-2 days from when we receive the sample at the lab.
How do I get started?You can ask your Lancaster DHIA technician on test day or call the office. If you are not a Lancaster DHIA customer, please call and we can arrange to have sample vials sent to you.
What Tests are performed?You may send a bulk tank sample or individual cows for 2 different tests. Listed below are the specific tests for each analysis.
Full Panel Analysis
(including all major coagulase-negative staphylococci)1. Staphylococcus aureus
2. Staphylococcus sp. -
3. Streptococcus agalactiae
4. Streptococcus dysgalactiae
5. Streptococcus uberis
6. Escherichia coli
7. Enterococcus sp. -
(including E. faecalis and E. faecium)8. Klebsiella sp. -
(including K. oxytoca and K. pneumoniae)9. Serratia marcescens
10. Corynebacterium bovis
11. Arcanobacter pyogenes and Peptoniphilus - (Peptostreptococcus) indolicus
12. Staphylococcal β-lactamase gene
Contagious Analysis
(Bulk Tank Screening)
1. Staphylococcus aureus
2. Streptococcus agalactiae
3. Mycoplasma bovis
Lancaster DHIA provides the following services in the Culture Lab:
PCR-based DNA Mastitis Testing
Mycoplasma testing
Hand Towel & Bedding Cultures
USDA Certified Johnes Milk ELISA TestingCosts of PCR-based Mastitis Testing
Lancaster DHIA Customer Price $24 Full Panel Test
$19 Contagious Test
Volume Discount over 30 samples
Non LDHIA Customer Price
$26 Full Panel Test
$21 Contagious Test
Volume Discount over 30 samples
Group Discounts for Milk Cooperatives
Shipping available to all areas in USA
All Pricing Agreements must be approved by Lancaster DHIA management